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KMID : 0545120040140030532
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 3 p.532 ~ p.539
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Effect of Carbon Source Consumption Rate on Lincomycin Production from Streptomyces lincolnensis
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Choi DB
Cho KA
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Abstract
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For efficient lincomycin production from Streptomyces lincolnensis L1245 various vegetable oils natural nitrogen sources and surfactants were investigated at the pilot-scale level in the flask. Olive oil as the sole carbon source was the most suitable one for producing lincomycin. When 20 g/l of olive oil was used the lincomycin concentration and lipase activity reached 1.01 g/l and 182 U/ml respectively after 5 days of culture. Among the various unsaturated fatty acids when linolenic acid was used the cell growth and lincomycin production were markedly decreased. On the other hand when 0.2 g/l of oleic acid was added to the culture broth the maximum lincomycin concentration was 1.0 g/l which was about 1.7-fold higher than that obtained without the addition of oleic acid. Among the various natural nitrogen sources pharmamedia or soybean meal was the most suitable nitrogen source. In particular in the case of a mixture of 10 g/l of pharmamedia and soybean meal 1.5 g/l of lincomycin concentration and 220 U/ml of lipase activity were obtained. When Span 180 was used as the surfactant lincomycin production lipase activity and oil consumption increased. The correlation between the consumption rates of oil and lincomycin production in a culture using olive oil as the sole carbon source was also investigated. The lincomycin production depended on the consumption rate of olive oil. Using these results fed-batch cultures for comparing the use of olive oil and starch as a conventional carbon source were carried out in a 5-l fermentor. When olive oil was used as the sole carbon source 34 g/l of olive oil was consumed after 7 days of culture. The maximum lincomycin concentration was 3.0 g/l which was about 2.0-fold higher than that of starch medium after 7 days of culture. The product yield was 0.09 g/g of consumed carbon source which was about 3.0-fold higher than that of starch medium after 7 days of culture.
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